Catalog Number : KT909

Kit Insert : KT909_SARS-Cov-2_Real-time RT-PCR_RT-qPCR_Detection_Kit

Description : SARS-CoV-2 Coronavirus (Covid-19) Real-Time RT-PCR (RT-qPCR) Detection Kit is designed to detect the presence of SARS-CoV-2 Coronavirus in respiratory specimens and serum sample as recommended by CDC (Centre for Diesease Control) Atlanta, USA.

Background : Coronaviruses are a family of large RNA viruses with size ranging from 26 to 32 kb. These viruses are zoonotic and in human can cause respiratory infections. As the coronavirus is an RNA virus it has a relatively high mutation rate resulting in rapid evolution. In December 2019, a new deadly coronavirus known as SARSCoV-2 (previously known as 2019-nCoV), which has a high sequence similarity to SARS-CoV, was identified and has caused a pneumonia, known as Covid-19, outbreak in Wuhan, China and spread globally.

Assay Principle : This kit contain two primer/probe sets (N gene primer/probe set 1, N gene primer/probe set 2) that target the conservative regions of coronavirus nucleocapsid (N) gene, the Human RPP30 gene primer/probe set (Human RPP30 gene primer/probe set) that targets exon 1 of human RPP30 gene and serves as a control to assess specimen quality, a non-infectious DNA positive control (Positive control (non-infectious DNA) to ensure reagents and instruments are working properly, and a negative human specimen extraction control (human RNA extract from non-infected samples, Human specimen extraction control) for assessing reverse transcription.
Refer to the datasheet for all details.